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SEQXE

Sigma-Aldrich

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Synonyme(s) :

SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit

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About This Item

Code UNSPSC :
41121800
Nomenclature NACRES :
NA.55

Technique(s)

whole genome amplification: suitable

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Catégories apparentées

Description générale

SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.

Application

SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.

Caractéristiques et avantages

  • Random priming technology amplifies fragmented DNA such as ChIP or FFPE
  • Facilitates sequencing from as little as 100 pg of ChIP DNA
  • Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
  • Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing

Autres remarques

SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.

Informations légales

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
iCAT is a registered trademark of University of Washington

Composants de kit également disponibles séparément

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Description
FDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologyFDS
  • Library Preparation Buffer

En option

Réf. du produit
Description
Tarif

Nécessaire, mais non fourni

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.
Mian S A, et al.
Nature Communications, 6, 10004-10004 (2015)
Syuzo Kaneko et al.
Cancers, 13(9) (2021-05-01)
Although chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) using formalin-fixed paraffin-embedded tissue (FFPE) has been reported, it remained elusive whether they retained accurate transcription factor binding. Here, we developed a method to identify the binding sites of the insulator transcription factor
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Protocoles

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

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