The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.
MOD50
Kit de modification de l'ADN Imprint®
For bisulfite DNA conversion & purification
Synonyme(s) :
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
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595.00 CHF
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595.00 CHF
About This Item
Produits recommandés
Utilisation
sufficient for 50 reactions
Température de stockage
20-25°C
Catégories apparentées
Description générale
The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Caractéristiques et avantages
- Only 50 picograms of DNA or 20 cells are required
- Procedure takes less than 2 hours
- Greater than 99% conversion rate
- Extremely low degradation
- Option of convenient one-step protocol
- Consistent and reproducible Bisulfite Modification
- Can be used with genomic, endonuclease digested, and FFPE DNA
Stockage et stabilité
All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.
Informations légales
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalFDS
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Risques supp
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
Histone H3 Lysine 36 Trimethylation Is Established over the Xist Promoter by Antisense Tsix Transcription and Contributes to Repressing Xist Expression
Ohhata T, et al.
Molecular and Cellular Biology null
MicroRNA-20 induces methylation of hepatitis B virus covalently closed circular DNA in human hepatoma cells
In Young Moon, et al.
Molecular Medicine Reports, 2285-2293 (2019)
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
Articles
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
Protocoles
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Contenu apparenté
The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.
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Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?
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What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?
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DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.
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How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?
1 answer-
This kit can be used for 50 reactions.
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Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?
1 answer-
Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.
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Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?
1 answer-
Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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