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M4071

Sigma-Aldrich

MINK1 (1-320), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinónimos:

B55, Hmink, MAP4K6, MGC21111, YSK2, ZC3, hMINKbeta

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

16-22 nmol/min·mg

mol wt

~61 kDa

UniProt accession no.

Q8N4C8

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MINK1(50488)

General description

Misshapen/NIKs-related kinase 1 (MINK1), also known as hMNIKβ or MINK is mapped to human chromosome 17p13.1. The gene codes for serine/threonine kinase, which is a member of the germinal center family of kinases (GCK) that are homologous to the yeast sterile 20 (Ste20) kinase family.

Biochem/physiol Actions

Misshapen/NIKs-related kinase 1 (MINK1) plays a vital role in modulating various cellular process such as actin cytoskeleton rearrangement, cell matrix adhesion, cell-cell adhesion, cell morphology, and survival. It is also implicated in the cell motility and cell invasion. Kinase inactive mutant of MINK1 increased cell spreading, actin stress fiber formation, adhesion to extracellular matrix and inhibited cell motility and invasion. Thus, alteration in the MINK1 activity inhibits tumor development. MINK is activated after Ras induction via a method involving reactive oxygen species (ROS). This activated MINK triggers the activity of p38 stress-activated kinase via MAP3K (Mitogen-Activated Protein Kinase Kinase Kinase) 5/8 and MKK (mitogen-activated protein kinase kinase) 3/6, which leads to p53-independent induction of cyclin-dependent kinase inhibitor, p21WAF1/CIP1 and permanent cell cycle arrest. MINK1 has an essential role in abscission, which is a final stage of cytokinesis.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

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Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis.
Hyodo T
The Journal of Biological Chemistry, 287(30), 25019-25029 (2012)
Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells.
Nicke B
Molecular Cell, 20(5), 673-685 (2005)
Identification and functional characterization of a novel human misshapen/Nck interacting kinase-related kinase, hMINK beta.
Hu Y
The Journal of Biological Chemistry, 279(52), 54387-54397 (2004)
H Ozcelik
Cloning and Characterization of Expanded CAG-Repeat Containing Sequence(s): Identification of Candidate Breast Cancer Gene(s). (2003)

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