This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
EC888
SmartLyse® Viral Buffer
Lysis buffer for viral RNA and DNA purification. 500ML.
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UNSPSC Code:
41105600
NACRES:
NB.23
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Servicio técnico
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Permítanos ayudarleGeneral description
GenElute™-E purification kits provide a simple alternative for viral RNA and DNA purification. Compared with traditionalmethods, GenElute™-E offers:
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, and subject the nucleic acids to centrifugation sheering forces.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in swab, cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminantswhile allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-qualitypurification is made possible by our innovative SmartLyse® Buffer, which enables fast and efficient isolation.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply prepare spin column, mix sample with lysis components, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified viral RNA and DNA is ready to be used in downstream applications or stored.
- Isolation of viral RNA and DNA in less than 30 minutes with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, and subject the nucleic acids to centrifugation sheering forces.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in swab, cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminantswhile allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-qualitypurification is made possible by our innovative SmartLyse® Buffer, which enables fast and efficient isolation.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply prepare spin column, mix sample with lysis components, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified viral RNA and DNA is ready to be used in downstream applications or stored.
Application
Suitable for most common downstream applications, including genotyping, PCR, and NGS
The GenElute™-E Viral RNA/DNA Swab kit has been developed to purify genomic viral RNA and DNA from nasopharyngeal swabs, dry or in transport medium, in addition to genital swabs and stool samples.
The sample lysis utilizes the SmartLyse® Viral Buffer for fast and efficient isolation of nucleic acids from a variety of swab types and stool samples.
Purified viral RNA and DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
The sample lysis utilizes the SmartLyse® Viral Buffer for fast and efficient isolation of nucleic acids from a variety of swab types and stool samples.
Purified viral RNA and DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
Features and Benefits
- Fast and efficient purification: no lysis incubation and minimal hands-on, preparation steps
- Higher quality nucleic acids: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents
- Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. The single-spin protocol limits the number of collection and pipetting steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GeGenElute™-E is designated a Greener Alternative Product based on the ""Prevention"" and ""Designing Safer Chemicals"" qualifications of The 12 Principles of Green Chemistry. GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
SmartLyse is a registered trademark of Merck KGaA, Darmstadt, Germany
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| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| input RNA | input sample(s) (swabs: nasopharyngeal and genital) | input DNA | input DNA |
signalword
Danger
supp_hazards
Clase de almacenamiento
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Corr. 1C
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 answer-
Helpful?
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 answer-
The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 answer-
Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
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Does the technology introduce any bias into the sample?
1 answer-
GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
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