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B7014

Anti-Goat IgG (whole molecule)–Biotin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
19


biological source

rabbit

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:20,000

storage temp.

−20°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.
Goat IgG is often used for the detection and study of various proteins such as EPAS1 and MIP-3 α among several others. Anti-goat IgG (whole molecule)-Biotin antibody can be used as a secondary antibody to facilitate the detection of such target proteins bound to goat IgGs by immunological assays . Thus, anti-goat IgGs are useful tools for the analysis of target proteins. Anti-Goat IgG (whole molecule)-Biotin antibody is specific for IgG in goats.

Immunogen

Goat IgG

Application

Anti-Goat IgG (whole molecule)-Biotin antibody produced in rabbit has been used:
  • immunohistochemistry
  • immunostaining
  • western blot
  • immunofluorescence
  • immunohistochemical studies

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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A5670B7389B8895
technique(s)

direct ELISA: 1:20,000

technique(s)

direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)

technique(s)

direct ELISA: 1:20,000

technique(s)

direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)

conjugate

biotin conjugate

conjugate

peroxidase conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

goat

biological source

goat

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution


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Hausmann M, et al.
Journal of immunology (Baltimore, Md. : 1950), 175(3), 1389-1398 (2005)
Propulsive appliance stimulates the synthesis of insulin-like growth factors I and II in the mandibular condylar cartilage of young rats
Hajjar D, et al.
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Comparative characterization of genome-wide transcriptional changes during infection can help elucidate the mechanisms underlying host susceptibility. In this study, transcriptional profiling of the mouse colon was carried out in two cognate lines of mice that differ in their response to



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SKUGTIN
B7014-.5ML04061833441527

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