Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as placental transport, complement fixation and phagocytosis among many others. The antigen binding is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain , . Anti-human IgG (γ-chain specific), F(ab′)2 fragment-alkaline phosphatase antibody can be used in ELISA for measuring anticardiolipin antibodies. Goat anti-human IgG (γ-chain specific), F(ab′)2 fragment-alkaline phosphatase antibody reacts specifically with human IgG.
Immunogen
Purified human IgG.
Application
Serum IgGs against HHV-6 or HHV-7 were detected by Elisa using alkaline phosphatase conjugated goat anti-human IgG F′ab specific at a concentration of 1:1000 diluted in PBS/0.05% skim milk. The antibody was incubated on plates for 2 hours at 37 degrees.
Transfected SN56 neuronal cell lysates were ran on a SDS page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG Fab specific as the secondary antibody.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
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