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86C

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast blue BB salt

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UNSPSC Code:
12352106
NACRES:
NA.47
eCl@ss:
42010105
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Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

dilution

(for histology)

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast blue BB. The resulting insoluble diffuse, blue dye deposit indicates sites of alkaline phosphatase activity.


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Solo componentes del kit

Referencia del producto
Descripción

  • Citrate Solution (915) 50 mL

  • FBB-Alkaline Solution (863) 10 mL

  • Naphthol AS-BI Alkaline Solution (861) 10 mL

  • Neutral Red Solution (N6264) 50 mL

  • Sodium Nitrite Solution (914) 10 mL

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

wgk

WGK 3

Clase de almacenamiento

10 - Combustible liquids



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Gregory M Cooper et al.
Tissue engineering. Part A, 16(5), 1749-1759 (2009-12-24)
The purpose of this study was to demonstrate spatial control of osteoblast differentiation in vitro and bone formation in vivo using inkjet bioprinting technology and to create three-dimensional persistent bio-ink patterns of bone morphogenetic protein-2 (BMP-2) and its modifiers immobilized
Ming-Tzu Tsai et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 27(9), 1169-1174 (2009-03-11)
Human mesenchymal stem cells (hMSCs) are a promising candidate cell type for regenerative medicine and tissue engineering applications by virtue of their capacity for self-renewal and multipotent differentiation. Our intent was to characterize the effect of pulsed electromagnetic fields (PEMFs)
S A Kuznetsov et al.
The Journal of cell biology, 153(5), 1133-1140 (2001-05-31)
We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, "mesenchymal



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SKUGTIN
86C-1KT04061833042328

Questions

  1. Can the 86C or 86R kits be used to detect Alkaline Phosphatase in cell culture media?

    1 answer
    1. The 86C and 86R kits are intended for use with adherent cells. The same applies to SCR004. The APF kit, a fluorescence-based kit, would be more suitable for use if trying to detect alkaline phosphatase in culture media.

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