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45245

Eosin 5-isothiocyanate

≥95.0% (UV)

Sinónimos:

EITC

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Fórmula empírica (notación de Hill):
C21H7Br4NO5S
Número CAS:
Peso molecular:
704.96
UNSPSC Code:
12352200
PubChem Substance ID:
MDL number:


assay

≥95.0% (UV)

fluorescence

λex 521; λem 544 (pH 9.0)

storage temp.

2-8°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S

InChI

1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)

InChI key

MCXYFYWNOWPDMA-UHFFFAOYSA-N

Application

Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.

Other Notes

Acceptor fluorophore. Measurement of rotational diffusion of proteins; In fluorescence energy transfer studies[1][2]


pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

flash_point_f

Not applicable

hcodes



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S Q Liu et al.
The American journal of physiology, 264(5 Pt 1), C1155-C1164 (1993-05-01)
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Ryan R Hansen et al.
Analytical and bioanalytical chemistry, 392(1-2), 167-175 (2008-07-29)
Quantitative evaluation of minimal polynucleotide concentrations has become a critical analysis among a myriad of applications found in molecular diagnostic technology. Development of high-throughput, nonenzymatic assays that are sensitive, quantitative and yet feasible for point-of-care testing are thus beneficial for
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The Journal of cell biology, 126(4), 901-910 (1994-08-01)
A simple method is described for high-resolution light and electron microscopic immunolocalization of proteins in cells and tissues by immunofluorescence and subsequent photooxidation of diaminobenzidine tetrahydrochloride into an insoluble osmiophilic polymer. By using eosin as the fluorescent marker, a substantial



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