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PC386

PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb

Name: PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

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NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

rat, mouse, human

Application:

—

Citations:

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Propiedades

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

rat, mouse, human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(Immunoblotting (1:1000)
Immunoprecipitation (1:200))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer15)

Gene Information

human ... TP53(7157)

Descripción

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser¹⁵.

Recognizes the ~53 kDa p53 protein phosphorylated at Ser¹⁵ in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.

This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH

Application

Immunoblotting (1:1000)
Immunoprecipitation (1:200)

Physical form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Analysis Note

Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents

Other Notes

Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.

Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Este artículo	PC712	OP03	OP33
Sigma-Aldrich PC386 PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb	Sigma-Aldrich PC712 Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb	Sigma-Aldrich OP03 Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)	Sigma-Aldrich OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
antibody form affinity isolated antibody	antibody form purified antibody	antibody form purified antibody	antibody form purified antibody
storage temp. −20°C	storage temp. −70°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
clone polyclonal	clone polyclonal	clone PAb421, monoclonal	clone PAb1620, monoclonal

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wgk

WGK 2

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SKU	GTIN
PC386-50UL	04055977224559

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