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KCQS04

KiCqStart® Probe qPCR ReadyMix

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

Sinónimos:

Kicqstart® Probe qPCR ReadyMix

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Tamaño de envaseSKUDisponibilidadPrecio
250 reactions
Comprobar disponibilidad del carrito
$243.00
1250 reactions
Comprobar disponibilidad del carrito
$1,080.00

Acerca de este artículo

NACRES:
NA.55
UNSPSC Code:
41106300

$243.00


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form

liquid

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

feature

dNTPs included, hotstart

storage condition

protect from light

technique(s)

RT-qPCR: suitable, qPCR: suitable

color

light blue

input

purified DNA

compatibility

Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2 , Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex , Eppendorf® Mastercycler ep realplex2 S, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480

detection method

probe-based

shipped in

dry ice

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Este artículo
KCQS05KCQS06KCQS07
technique(s)

RT-qPCR: suitable

technique(s)

RT-qPCR: suitable

technique(s)

RT-qPCR: suitable

technique(s)

RT-qPCR: suitable

form

liquid

form

liquid

form

liquid

form

liquid

usage

sufficient for 1250 reactions, sufficient for 5000 reactions, sufficient for 250 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 500 reactions

feature

hotstart, dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

compatibility

Bio-Rad CFX384, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 S, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene Q, Bio-Rad CFX96, Bio-Rad MiniOpticon, Illumina Eco qPCR, Roche LightCycler 480 , Bio-Rad MJ Opticon 2 , Qiagen Corbett Rotor-Gene 6000, Eppendorf® Mastercycler ep realplex

compatibility

ABI 7500 Fast, ABI QuantStudio, ABI ViiA 7 , Agilent AriaMx, Douglas Scientific IntelliQube, Qiagen Corbett Rotor-Gene Q, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000

compatibility

ABI 5700, ABI 7000, ABI 7300, ABI 7700, ABI 7900 HT Fast, ABI StepOne, ABI StepOnePlus

compatibility

Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MiniOpticon, Eppendorf® Mastercycler ep realplex2 s, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

KiCqStart® Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.

Features and Benefits

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Other Notes

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

Eppendorf is a registered trademark of Eppendorf SE
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

related product

Referencia del producto
Descripción
Precios

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Disruption of KIF3A in patient-derived glioblastoma cells: effects on ciliogenesis, hedgehog sensitivity, and tumorigenesis
Lan B
Oncotarget (2016)

Artículos

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

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Protocolos

KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.

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