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Merck

T4019

Sigma-Aldrich

Trypsin−Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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About This Item

Número MDL:
Código UNSPSC:
12352204
eCl@ss:
42020142
NACRES:
NA.54

origen biológico

bovine pancreas (trypsin)

formulario

buffered aqueous suspension

concentración

≥15 units/mL (packed gel)

Extensión del etiquetado

≥15 units per mL (packed gel)

matriz

beaded agarose

temp. de almacenamiento

2-8°C

Descripción general

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Aplicación

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

Definición de unidad

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Forma física

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

Nota de preparación

TPCK-treated

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Journal of virology, 86(17), 9465-9473 (2012-06-29)
The high-risk human papillomavirus type 16 (HPV-16) E5 protein (16E5) induces tumors in a transgenic mouse model and may contribute to early stages of cervical carcinogenesis. Although high-risk E5 expression is generally thought to be lost during the progression to
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