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T1763

Trypsin Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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A ustedes/SKUDisponibilidadPrecio
25 units
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96,20 €
50 units
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168,00 €

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UNSPSC Code:
12352204
eCl@ss:
42020142
NACRES:
NA.54
Número CE:
3.4.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00082094
Biological source:
bovine pancreas (trypsin)
Concentration:
≥15 units/mL (packed gel)

96,20 €

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biological source

bovine pancreas (trypsin)

Quality Segment

200

form

buffered aqueous suspension

concentration

≥15 units/mL (packed gel)

extent of labeling

≥15 units per mL packed gel

matrix

cross-linked beaded agarose

shipped in

wet ice

storage temp.

2-8°C

General description

Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.
A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. [1] Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography. [2]

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2

Other Notes

Insolubilized
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

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Este artículo
T4019C1261T8802
Trypsin Agarose buffered aqueous suspension, from bovine pancreas trypsin

Sigma-Aldrich

T1763

Trypsin Agarose

Trypsin−Agarose buffered aqueous suspension, from bovine pancreas trypsin

Sigma-Aldrich

T4019

Trypsin−Agarose

Carboxypeptidase A−Agarose ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

Sigma-Aldrich

C1261

Carboxypeptidase A−Agarose

Tripsina from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Sigma-Aldrich

T8802

Tripsina from bovine pancreas

biological source

bovine pancreas (trypsin)

biological source

bovine pancreas (trypsin)

biological source

enzyme from bovine pancreas

biological source

bovine pancreas

form

buffered aqueous suspension

form

buffered aqueous suspension

form

ammonium sulfate suspension

form

essentially salt-free, lyophilized powder

concentration

≥15 units/mL (packed gel)

concentration

≥15 units/mL (packed gel)

concentration

-

concentration

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

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