P4306

Platelet-Derived Growth Factor-BB human

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinónimos: PDGF-BB
• Número MDL: MFCD00213023
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Análisis: ≥97% (SDS-PAGE)
• formulario: lyophilized powder
• potencia: 1.0-3.0 ng/mL EC₅₀
• calidad: endotoxin tested
• mol peso: 24.3 kDa
• envase: pkg of 10 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤1 EU/μg
• Nº de acceso UniProt: P01127
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... PDGFB(5155)

Descripción

Descripción general

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin. PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Aplicación

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.

Acciones bioquímicas o fisiológicas

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis. PDGF-BB controls bone homeostasis, repair and regeneration. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

Otras notas

Dimer of the B chain of human PDGF.

Forma física

Lyophilized from a 0.2 μm filtered solution.

Nota de análisis

The biological activity is measured by the dose-dependent stimulation of thymidine uptake in BALB/c 3T3 cells.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)