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P3201

Sigma-Aldrich

Platelet-Derived Growth Factor-BB human

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinónimos:

PDGF-BB

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About This Item

Número MDL:
MFCD00213023
Código UNSPSC:
12352202
NACRES:
NA.77

origen biológico

human

Nivel de calidad

200

recombinante

expressed in E. coli

Análisis

≥98% (HPLC and SDS-PAGE)

formulario

lyophilized powder

potencia

1.0-3.0 ng/mL EC50

calidad

endotoxin tested

mol peso

~25 kDa

envase

pkg of 10 μg
pkg of 50 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

técnicas

cell culture | mammalian: suitable

impurezas

≤1 EU/μg

Nº de acceso UniProt

P01127

temp. de almacenamiento

−20°C

Información sobre el gen

human ... PDGFB(5155)

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Descripción general

Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.

Aplicación

Platelet-Derived Growth Factor-BB human has been used :
  • As a growth supplement in proximal tubule and glomerular explant cell culture
  • In three-dimensional scaffold preparation
  • To study the effect of brivanib on growth factor signaling pathways
  • In transwell migration assay

Acciones bioquímicas o fisiológicas

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.

Otras notas

Dimer of the B chain of human PDGF.

Forma física

Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.

Nota de análisis

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis.
Kohannim O,et al.
Case Reports in Genetics, 2011 , 1-6 (2011)
Matrix stiffness regulates migration of human lung fibroblasts.
Asano1 S, et al.
Physiological Reports, 5.9, 1-11 (2017)
Yasutoshi Takashima et al.
American journal of physiology. Renal physiology, 310(5), F395-F408 (2016-01-15)
Glomerular integrity and functions are maintained by growth factor signaling. Heparan sulfate, the major component of glomerular extracellular matrixes, modulates growth factor signaling, but its roles in glomerular homeostasis are unknown. We investigated the roles of heparan sulfate 6-O-endosulfatases, sulfatase
Injury induces in vivo expression of platelet-derived growth factor (PDGF) and PDGF receptor mRNAs in skin epithelial cells and PDGF mRNA in connective tissue fibroblasts
Antoniades HN
Proceedings of the National Academy of Sciences of the USA, 88.2, 565-569 (1991)
Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling.
Nakamura I, et al.
PLoS ONE, 9.4 (2014)
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