indirect ELISA: 1:5,000 using mouse primary antibody, bridging antibody and horseradish peroxidase
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal anti-peroxidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen
horseradish peroxidase
Application
Monoclonal Anti-Peroxidase antibody may be used for:
immunocytochemistry
indirect ELISA (1:5,000)
immunohistochemistry
immunoblotting
Biochem/physiol Actions
Peroxidase is often used as an enzyme label for antibodies during analyses of target antigens. Peroxidases aids in substrate conversion indicated by the formation of a coloured product. In an alternative procedure, an anti-enzyme antibody can be used to bridge a primary antibody and enzyme complex for the study of target proteins. Anti-peroxidase antibodies are also used to prepare peroxidase-anti-peroxidase (PAP) soluble complexes. Monoclonal Anti-Peroxidase antibody can be used for signal amplification in a wide range of immunological techniques. The product is specific for horseradish peroxidase. Such immunoassays are useful tools to detect antigens, toxins, bacteria and viruses.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
