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N6635

Nutrient Mixture F-10 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Ham’s F-10

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UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217607
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form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

General description

Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues.

Application

Nutrient Mixture F-10 Ham has been used:

  • for the in vitro tissue culture from rabbit[1]
  • to culture satellite cells from fish white muscle of trout[2]
  • for culturing neonatal porcine islets (NPIs)[3]

Preparation Note

Formulated to contain 9.8 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.

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Este artículo
N6013N2147N6908
Sigma-Aldrich

Sigma-Aldrich

N6635

Nutrient Mixture F-10 Ham

Nutrient Mixture F-10 Ham With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

N6013

Nutrient Mixture F-10 Ham

Nutrient Mixture F-10 Ham With 20 mM HEPES, without sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

N2147

Nutrient Mixture F-10 Ham

Nutrient Mixture F-10 Ham With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

N6908

Nutrient Mixture F-10 Ham

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

liquid

form

liquid

form

liquid

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

components

HEPES: no
phenol red: yes
L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes

components

HEPES: 20 mM
L-glutamine: no
sodium pyruvate: 0.11 g/L
phenol red: yes
NaHCO3: no

components

L-glutamine: yes
HEPES: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes
phenol red: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Low serum media

Human diploid cell culture, low serum

Human diploid cell culture, low serum

Low serum media

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Preguntas

  1. While preparing media solution from nutrient mixture F-10 Ham powder, is it recommended to add antifungal and antibiotic in media solution? If so, what amount of it should be added in 1L of media?

    1 respuesta

    1. This product does not contain antibiotics or antifungals by default. The addition of these constituents is at the discretion of the end user. The final concentration is dependent upon the type of product used – penicillin, streptomycin, etc. Several examples are listed below:
      P0781, Penicillin-Streptomycin, 10,000 units penicillin and 10 mg streptomycin per mL, add 10 mL per liter medium
      https://www.sigmaaldrich.com/product/sigma/p0781

      P4458, Penicillin-Streptomycin, 5,000 units penicillin and 5 mg streptomycin per mL, add 20 mL per liter medium
      https://www.sigmaaldrich.com/product/sigma/p4458

      A5955, Penicillin-Streptomycin-Amphotericin B Suspension, Stabilized, with 10,000 units penicillin, 10 mg streptomycin and 25 ?g amphotericin B per mL, add 10 mL per liter medium
      https://www.sigmaaldrich.com/US/en/product/sigma/a5955

      N1638, Nystatin Suspension, suspension, 10,000 unit/mL, add 10 mL per liter medium
      https://www.sigmaaldrich.com/product/sigma/n1638

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