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D8437

DMEM/F-12

With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Sinónimos:

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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Tamaño de envaseSKUDisponibilidadPrecio
500 mL
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29,20 €

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UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417

29,20 €

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Nombre del producto

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Dulbecco′s Modified Eagle′s medium (DMEM), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids.[1] A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:
  • has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells[1]
  • has been used to culture the cartilage biopsies[2]
  • may be used for the propagation of Cowdria ruminantium[3]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Este artículo
D8062D8900D6434
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D8437

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D8062

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6434

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

form

liquid

form

liquid

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

components

L-glutamine: no
phenol red: no
NaHCO3: yes
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBM0553
RNBL9772
RNBL7914
RNBL7691
RNBL5998

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Artículos

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

Chris M Valdez et al.
Scientific reports, 7(1), 16372-16372 (2017-11-29)
A bipolar (BP) nanosecond electric pulse (nsEP) exposure generates reduced calcium influx compared to a unipolar (UP) nsEP. This attenuated physiological response from a BP nsEP exposure is termed "bipolar cancellation" (BPC). The predominant BP nsEP parameters that induce BPC
Iago Méndez-López et al.
Journal of hypertension, 35(8), 1594-1608 (2017-04-14)
It has been suggested that Ca entry through store-operated Ca channels (SOCs) is regulated by a dynamic interplay between the endoplasmic reticulum Ca stores and the mitochondria. These relationships drive the activation and inactivation of SOCs, yet it remains unclear
Višnja Stepanić et al.
Antioxidants (Basel, Switzerland), 8(4) (2019-04-10)
Chalcones are polyphenolic secondary metabolites of plants, many of which have antioxidant activity. Herein, a set of 26 synthetic chalcone derivatives with alkyl substituted pyrazine heterocycle A and four types of the monophenolic ring B, were evaluated for the potential
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SKUGTIN
D8437-500ML04061833589649
D8437-24X500ML04061833589632
D8437-6X500ML04061833589656
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Preguntas

1–7 de 7 Preguntas  

  1. Dear, Can you tell me the composition of this medium, or at least the amount of L-Glutamine present in this media? Kind regards, Sarah

    1 respuesta

    1. For a list of components, see the link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/747/307/d8437for.pdf

      ¿Le ha resultado útil?

  2. Is the glutamine that has already been added stable glutamine? How long is the shelf life?

    1 respuesta

    1. This product does not contain Alanyl-glutamine, which is considered a more stable form It has been supplemented with L-glutamine and will remain stable through out the shelf life of the product with proper storage and handling. Typical lots of D8437 are assigned an expiration date of 1 year from the date of release. Note that under culture conditions of 37 deg C the L-glutamine will gradually degrade. See the link below to review general stability information regarding L-glutamine.:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/195/924/t075.pdf

      Alanyl-glutamine is also available as a sterile medium supplement. Please see the link below for an example of one cell culture product option:
      https://www.sigmaaldrich.com/product/sigma/g8541

      ¿Le ha resultado útil?

  3. Can Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, be used under serum free conditions?

    1 respuesta

    1. When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).

      ¿Le ha resultado útil?

  4. What is the storage temperature of Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    1 respuesta

    1. We recommend storing Product No. D8437 at 2-8°C.

      ¿Le ha resultado útil?

  5. What is Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    1 respuesta

    1. This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).

      ¿Le ha resultado útil?

  6. What is the Department of Transportation shipping information for this product?

    1 respuesta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      ¿Le ha resultado útil?

  7. What is the optimal carbon dioxide concentration needed for Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    1 respuesta

    1. This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.

      ¿Le ha resultado útil?

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