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D8437

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, sterile liquid, suitable for cell culture

Sinónimos:

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

Número MDL:
MFCD00217417
Código UNSPSC:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

esterilidad

sterile-filtered

formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Dulbecco′s Modified Eagle′s medium (DMEM), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.

Aplicación

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:
  • has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells
  • has been used to culture the cartilage biopsies
  • may be used for the propagation of Cowdria ruminantium

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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