D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

• Sinónimos: DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
• Número MDL: MFCD00217417
• Código UNSPSC: 12352207
• NACRES: NA.75

Propiedades

• product name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
• esterilidad: sterile-filtered
• formulario: liquid
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• componentes: glucose: 3.15 g/L; NaHCO₃: yes; L-glutamine: yes; phenol red: yes; HEPES: no; sodium pyruvate: 0.055 g/L
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Aplicación

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

• to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites
• to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment
• to culture mouse squamous cell carcinoma (SCCVII) tumor cells

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable