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D7291

Sigma-Aldrich

Deoxyribonuclease I RNase-free solution from bovine pancreas

Sinónimos:

DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I

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About This Item

Número de CAS:
9003-98-9
Comisión internacional de enzimas:
3.1.21.1 (BRENDA, IUBMB)
Número CE:
232-667-0
Número MDL:
MFCD00130918
Código UNSPSC:
12352204
NACRES:
NA.53

origen biológico

bovine pancreas

grado

for molecular biology

formulario

buffered aqueous glycerol solution

actividad específica

10000 units/mg protein





mol peso

29.1 kDa

concentración

5—15 mg protein/mL

técnicas

DNA extraction: suitable

idoneidad

suitable for molecular biology

Nº de acceso UniProt

P00639

aplicaciones

cell analysis

actividad extraña

RNase, none detected

temp. de almacenamiento

−20°C

Información sobre el gen

cow ... DNASE1(282217)

Descripción general

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Aplicación

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Componentes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Definición de unidad

One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Otras notas

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

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Ribonucleasa A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

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Proteasa from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

DNA Ligation Kit

AMPD1

DNase I, Amplification Grade

inhibidor

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
Umesh Kumar Dhawan et al.
Arteriosclerosis, thrombosis, and vascular biology, 41(10), 2598-2615 (2021-08-06)
Objective: Hypercholesterolemia-induced NETosis and accumulation of neutrophil extracellular traps (NETs) in the atherosclerotic lesion exacerbates inflammation and is causally implicated in plaque progression. We investigated whether hypercholesterolemia additionally impairs the clearance of NETs mediated by endonucleases such as DNase1 and
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
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Protocolos

Deoxyribonuclease I

Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.

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