This product is a 2 mg/mL solution of Doxorubicin in pure DMSO. The material has not been specifically test for use in TNBC cells. In general, the final DMSO concentrations in cell culture medium should not exceed 0.5%. This product is prepared using Doxorubicin HCl with a molecular weight of 579.98. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/281/732/d5220pis-rev0922-ms.pdf
D5220
Doxorubicin Ready-Made
2mg/ml, Suitable for cell culture
Sinónimos:
Adriamycin, DOX, Hydroxydaunorubicin hydrochloride
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 134,00 € |
Acerca de este artículo
NACRES:
NA.76
UNSPSC Code:
12352200
134,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleQuality Segment
form
liquid
mol wt
579.98 Da
packaging
pkg of 1 mL
concentration
2 mg/mL
shipped in
dry ice
storage temp.
-10 to -25°C
Biochem/physiol Actions
Doxorubicin is an antitumor antibiotic that acts by inhibiting DNA topoisomerase II. It intercalates with DNA and induces apoptosis by blocking replication.
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Este artículo | |||
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| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level - |
| form liquid | form powder | form powder | form - |
| concentration 2 mg/mL | concentration - | concentration - | concentration - |
| storage temp. -10 to -25°C | storage temp. 2-8°C | storage temp. -10 to -25°C | storage temp. - |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| mol wt 579.98 Da | mol wt - | mol wt - | mol wt - |
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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I am considering using this ready made solution for my cellular assays aiming to induce senescence into TNBC cell lines. I am wondering what the molarity is for this ready made product and if there are any concerns surrounding the volume of DMSO?
1 respuesta-
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What is the molecular formula of the doxorubicin HCl used in this solution?
1 respuesta-
The Doxorubicin HCl used to prepare this product has a molecular formula of C27H29NO11 · HCl. See product D1515 at the link below for additional details:
https://www.sigmaaldrich.com/product/sigma/d1515¿Le ha resultado útil?
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la solution de "doxorubicin ready-made" (ref D5220) est diluée dans un buffer: quelle est sa composition ? (sels de phosphates ? ou autre)
1 respuesta-
This readymade solution contains doxorubicin hydrochloride at a concentration of 2 mg/mL in DMSO. Further information is available under "More Documents" or at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/281/732/d5220pis-rev0922-ms.pdf.
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When this substance is diluted and stored at -10°C to -25°C, what is the recommended storage duration?
1 respuesta-
This product is stable for at least 2 years in the undiluted state. Stability studies have not been performed on the material when diluted in aqueous solutions. Please see the link below to review a publication that may be helpful.
https://pubmed.ncbi.nlm.nih.gov/517541/¿Le ha resultado útil?
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What this product is dissolved in? Is it dissolved in water ?
1 respuesta-
This Ready-Made solution contains doxorubicin hydrochloride at a concentration of 2 mg/mL in DMSO. This solution may be diluted further with buffer or medium.
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