Pepsina from porcine gastric mucosa

Name: Pepsina from porcine gastric mucosa
Brand: SIGMA

powder, slightly beige, 1200-2400 U/mg

Sinónimos:

Pepsina A, Pepsina from hog stomach

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Número CAS:

9001-75-6

UNSPSC Code:

12352204

eCl@ss:

42010127

EC Number:

232-629-3

NACRES:

NA.54

MDL number:

MFCD00081840

Número CE:

3.4.23.1 (BRENDA, IUBMB)

Specific activity:

1200-2400 U/mg

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form

powder

specific activity

1200-2400 U/mg

mol wt

35 kDa

color

slightly beige

UniProt accession no.

P00791

storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

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Este artículo	77160	P7125	P6887
Sigma-Aldrich 77151 Pepsin from porcine gastric mucosa	Sigma-Aldrich 77160 Pepsin from porcine gastric mucosa	P7125 Pepsin from porcine gastric mucosa	P6887 Pepsin from porcine gastric mucosa
Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)
specific activity 1200-2400 U/mg	specific activity ≥500 U/mg	specific activity ≥400 units/mg protein	specific activity ≥3,200 units/mg protein
form powder	form powder	form powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. P00791
mol wt 35 kDa	mol wt 35 kDa	mol wt 35 kDa	mol wt 35 kDa

Application

Puede utilizarse hidrólisis con pepsina para producir los fragmentos F(ab′)₂ de los anticuerpos. pepsina en www.sigma-aldrich.com/enzymeexplorer

Biochem/physiol Actions

Escisión preferente: residuos hidrófobos y aromáticos en las posiciones P1 y P1′. Rompe los enlaces Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe y Phe-Tyr en la cadena β de la insulina

Analysis Note

pH óptimo es 2-4. Activa en urea 4 M y HCl de guanidina 3 M. Estable a 60 ºC. La pepsina se desactiva de manera irreversible a pH 8,0 - 8,5.

Other Notes

1 U corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37°C (Hemoglobin, Cat.No. 51290, as substrate) 15′000 absorbance-U as described above are equivalent to ∼1 Bergmeyer-U. 1 Bergmeyer-U is the amount of enzyme which hydrolyzes 1 μmol Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37°C

Disclaimer

Sales restrictions may apply.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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77151-5G	04061833627396

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