MABE251

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31

clone HMC 31, from mouse

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: HMC 31, monoclonal
• reactividad de especies (predicha por homología): all
• técnicas: ELISA: suitable; dot blot: suitable; methylated DNA immunoprecipitation (MeDIP): suitable
• isotipo: IgG1κ
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

Especificidad

This antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.

Inmunógeno

BSA-conjugated 5-hydroxymethylcytosine.

Epitope: 5-hydroxymethylcytosine

Aplicación

Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.

ELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.

Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Calidad

Evaluated by Dot Blot in unmodified and modified cytosine DNA.

Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.

Descripción de destino

This antibody detects 5-hydroxymethylcytosine.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Unmodified and modified cytosine DNA.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable