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Merck

71296

PhosphoSafe Extraction Reagent

Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

Sinónimos:

Protein Extraction Reagent

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NACRES:
NA.77
UNSPSC Code:
41106510

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Quality Level

100

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

immunoblotting: suitable

application(s)

sample preparation

shipped in

dry ice

storage temp.

−70°C

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Este artículo
71009-M7118670584-M
PhosphoSafe Extraction Reagent Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

Millipore

71296

PhosphoSafe Extraction Reagent

CytoBuster Protein Extraction Reagent Simple extraction of soluble protein from mammalian and insect cells

Millipore

71009-M

CytoBuster Protein Extraction Reagent

YeastBuster Protein Extraction Reagent Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis

Millipore

71186

YeastBuster Protein Extraction Reagent

BugBuster® Protein Extraction Reagent

Millipore

70584-M

BugBuster® Protein Extraction Reagent

technique(s)

immunoblotting: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

application(s)

sample preparation

application(s)

-

application(s)

-

application(s)

-

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage temp.

−70°C

storage temp.

10-30°C

storage temp.

-

storage temp.

10-30°C

General description





PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).

Features and Benefits

  • Phosphorylation state preserved during extraction
  • Compatible with kinase assays

Other Notes

Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Toxic (F)

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Pharmacologic ascorbate treatment (P-AscH-, high-dose, intravenous vitamin C) results in a transient short-term increase in the flux of hydrogen peroxide that is preferentially cytotoxic to cancer cells versus normal cells. This study examines whether an increase in hydrogen peroxide is

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Preguntas

1–2 de 2 Preguntas  

  1. what is recomended volume for 50mg tissue extraction? would you recomend adding protease ihnibitor too? could you share protocol for protein extraction from liver using this phosphosafe extraction reagent?

    1 respuesta

    1. A protocol for liver tissue particularly is not offered. Here is a protocol for tissue in general:

      Tissue homogenates are prepared by homogenizing tissue in ice-cold PhosphoSafe extraction buffer using 70 ul buffer/mg tissue. Homogenates are incubated at room temperature for 5 mins, sonicated on ice, and then subjected to centrifugation (spun at 16,000 X g, 5 mins, at 4 Celsius). The resulting supernatant is collected (the end-user will want to keep this, and can discard the insoluble pellet). Finally, protein concentration is determined by a protein assay (e.g., Bradford assay).

      To minimize proteolysis, extraction can be performed on ice, and protease inhibitors can be added.
      Please see the link below to access the full product user guide:
      https://www.emdmillipore.com/product/PhosphoSafe-Extraction-Reagent,EMD_BIO-71296#anchor_USP

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  2. I am doing protein isolation from the organic phase remaining after RNA isolation using Trizol/Chloroform. How can I use PhosphoSafe™ Extraction Reagent in this case?

    1 respuesta

    1. Phosphosafe has been tested for use in aqueous formats such as cell culture supernatants or lysates. The performance of the product in an organic phase following fractionation with Trizol has not been determined. To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

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