69725-M
λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
10 reactions | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 632,00 € |
Acerca de este artículo
NACRES:
NA.51
UNSPSC Code:
41106200
632,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarlemanufacturer/tradename
Novagen®
Quality Segment
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
storage temp.
−70°C
General description
The λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Other Notes
•200 µlλDE3 Phage Lysate
•200 µlHelper Phage Lysate
•200 µlSelection Phage Lysate
•50 µlT7 Tester Phage Lysate
•200 µlHMS174(DE3) glycerol stock
•1 µgpLysS DNA
•1 µgpLysE DNA
•200 µlHelper Phage Lysate
•200 µlSelection Phage Lysate
•50 µlT7 Tester Phage Lysate
•200 µlHMS174(DE3) glycerol stock
•1 µgpLysS DNA
•1 µgpLysE DNA
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
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Este artículo | |||
|---|---|---|---|
| storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 2
Certificados de análisis (COA)
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