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23-027-M

b-catenin Protein, activated, 10 µg

Name: b-catenin Protein, activated, 10 µg
Brand: MM
Price: 669 EUR

Activated, full length, recombinant human β-catenin. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using GSK-3a (Item # 14-492). For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

Sinónimos:

Catenin beta-1

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Tamaño de envase	SKU	Disponibilidad	Precio
10 μg		Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad	669,00 €
250 μg		Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad	3770,00 €

Acerca de este artículo

UNSPSC Code:

12352202

NACRES:

NA.41

eCl@ss:

32160405

Biological source:

human

Recombinant:

expressed in Sf21 cells

669,00 €

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Propiedades

biological source

human

Quality Level

100, 300

recombinant

expressed in Sf21 cells

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable

NCBI accession no.

NM_001904

Descripción

General description

β-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of β-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of β-catenin have been implicated in a number of human cancers.

N-terminal GST-tagged, recombinant human Smurf1 full length, expressed in E coli.

Application

Ubiquitination Cascade Comoponent: Substrate

Physical form

Purified using immobilized metal affinity chromatography.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este artículo	05-665-AF555	05-665-AF647	05-665-AF488
Sigma-Aldrich 23-027-M b-catenin Protein, activated, 10 µg	Sigma-Aldrich 05-665-AF555 Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor^™ 555	Sigma-Aldrich 05-665-AF647 Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor^™ 647	Sigma-Aldrich 05-665-AF488 Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor^™ 488
biological source human	biological source mouse	biological source mouse	biological source mouse
recombinant expressed in Sf21 cells	recombinant -	recombinant -	recombinant -
technique(s) activity assay: suitable	technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable	technique(s) flow cytometry: suitable, immunocytochemistry: suitable	technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
NCBI accession no. NM_001904	NCBI accession no. NP_001895	NCBI accession no. NP_001895	NCBI accession no. NP_001895
Quality Level 100, 300	Quality Level 100	Quality Level 100	Quality Level 100
manufacturer/tradename Upstate^®	manufacturer/tradename -	manufacturer/tradename -	manufacturer/tradename -

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Detecting Substrate Ubiquitination by the E3 Ligase, XIAP, Using Quantitative Assays

Quantitative assays for investigating ubiquitination pathways are essential for advancing ubiquitin-related drug discovery research. To illustrate the utility of in vitro ubiquitination enzyme cascades, we quantify the ubiquitination of Smac/DIABLO by the E3 ligase XIAP using a functional enzymatic reaction coupled to multiple assay readouts. We compare different detection methods that can be used to test small molecules for their potential to modulate XIAP-mediated ubiquitination in the context of this assay. The data provides a basis for use of these recombinant E3 ligase cascades in various applications, including screening campaigns.

?-Catenin signaling is a critical event in ErbB2-mediated mammary tumor progression.

Schade, Babette, et al.

Cancer Research, 73, 4474-4487 (2013)

Número de artículo de comercio global

SKU	GTIN
23-027M	04053252294433
23-027	04053252294723

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