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E0760

Endothelial cell growth supplement from bovine pituitary

Name: Endothelial cell growth supplement from bovine pituitary
Brand: SIGMA
Price: 197 CHF

Bioreagent, powder, suitable for cell culture

Synonyme(s) :

ECGS, Endothelial Mitogen

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Conditionnement	Référence	Disponibilité	Prix
15 mg		Vérifier la disponibilité des articles du panier	197.00 CHF

A propos de cet article

UNSPSC Code:

12352202

NACRES:

NA.77

Biological source:

bovine pituitary glands

Form:

powder

Impurities:

≤20 EU/vial (LAL test)

197.00 CHF

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Propriétés

Nom du produit

Endothelial cell growth supplement from bovine pituitary, ECGS, BioReagent, suitable for cell culture

biological source

bovine pituitary glands

Quality Segment

200

product line

BioReagent

form

powder

potency

2-300 μg/mL ED₅₀/EC₅₀

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

Description

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:

as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
as a supplement in medium 199 for endothelial cell culture
as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

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Cet article	E2759	P5208	E1388
Sigma-Aldrich E0760 Endothelial cell growth supplement from bovine pituitary	Sigma-Aldrich E2759 Endothelial cell growth supplement from bovine neural tissue	Sigma-Aldrich P5208 Platelet-Derived Endothelial Cell Growth Factor human	Sigma-Aldrich E1388 β-Endothelial Cell Growth Factor human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source bovine pituitary glands	biological source bovine neuroblastoma	biological source human	biological source human
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form powder	form lyophilized powder	form lyophilized powder
impurities ≤20 EU/vial (LAL test)	impurities ≤20 EU/vial (LAL test)	impurities <1 EU/mg	impurities ≤1.0 EU/μg protein Endotoxin level (LAL test)
storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)