ABC299
Anti-FFAR2 Antibody/GPR43
from rabbit, purified by affinity chromatography
Synonyme(s) :
Free fatty acid receptor 2, G-protein coupled receptor 43, FFAR2/GPR43
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat
Réactivité de l'espèce (prédite par homologie)
human (based on 100% sequence homology), mouse
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... FFAR2(2867)
Description générale
Free fatty acid receptor 2 (FFAR2), also known as G-protein coupled receptor 43 (GPR43), functions as the cell membrane receptor for short chain fatty acids that ultimately stimulates G protein coupled, inhibitory (Gi) signals via the adenylate cyclase pathway. FFAR2/GPR43 is found expressed in leukocytes in the lamina propria of the small intestine mostly but some expression can be also be found in splenocytes, adipose tissue, and enteroendocrine cells of the gut. Interesting, FFAR2/GPR43 because it senses short chain fatty acids which are often key metabolites of gut microbiota, it may serve as a biological sensor to regulate the activity of regulatory T cells and the immune response in diseases such as colitis.
Immunogène
Epitope: Cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human FFAR2/GPR43.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-FFAR2 Antibody/GPR43 is validated for use in Western Blotting and Immunohistochemistry for the detection of FFAR2/GPR43.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected FFAR2/GPR43 in 10 µg of RAW264.7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.
Qualité
Evaluated by Western Blotting in CHEM-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.
Description de la cible
~47 kDa observed. The calculated molecular weight of this protein is 37 kDa, but can be observed at ~47 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Nature communications, 9(1), 3674-3674 (2018-09-12)
Microbiome-derived metabolites influence intestinal homeostasis and regulate graft-versus-host disease (GVHD), but the molecular mechanisms remain unknown. Here we show the metabolite sensor G-protein-coupled receptor 43 (GPR43) is important for attenuation of gastrointestinal GVHD in multiple clinically relevant murine models. GPR43
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