Y4645

Anti-phospho-YAP1 (pTyr³⁵⁷) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-YAP, Anti-YAP2, Anti-YAP65, Anti-Yes-associated protein 1
• Número MDL: MFCD04118707
• Código UNSPSC: 12352203
• NACRES: NA.44

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~65 kDa
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• concentración: ~1.5 mg/mL
• técnicas: indirect immunofluorescence: 5-10 μg/mL using HEK-293 cells co-transfected with human YAP1 and c-Abl; western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and human c-Abl
• Nº de acceso UniProt: P46937
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: phosphorylation (pTyr357)
• Información sobre el gen: chicken ... YAP1(396171) ; human ... YAP1(10413) ; mouse ... Yap1(22601)

Descripción

Descripción general

Yes-associated protein 1 (YAP1) is a 65 kDa and a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with Src-family tyrosine kinase c-Yes. In addition to a SH3-binding motif, YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 binds to p73 through its WW domain and the PPPY motif of p73. This interaction is required for the ability of YAP1 to co-activate p73-responsive genes.

Especificidad

Anti-phospho-YAP1 pTyr³⁵⁷ antibody is specific for human phospho-YAP1 pTyr³⁵⁷. The product may react with chicken and mouse phospho-YAP1 pTyr³⁵⁷. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.

Inmunógeno

synthetic peptide corresponding to amino acids 351-362 (pTyr³⁵⁷) located in the C-terminal region of human YAP1. The sequence is identical in chicken YAP1 and highly conserved in mouse YAP1 (single amino acid substitution).

Aplicación

Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit has been used in western blotting and immunofluorescence.

Acciones bioquímicas o fisiológicas

Inhibition of Akt potentiates the nuclear re-localization of YAP1 to induce apoptosis by p73.

YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development
Anti-phospho-YAP1 pTyr³⁵⁷ antibody is specific for human phospho-YAP1 pTyr³⁵⁷. The product may react with chicken and mouse phospho-YAP1 pTyr³⁵⁷. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable