T5391

Apo-transferrina human

γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%

• Sinónimos: Siderofilina, Transferrina humana
• Número CAS: 11096-37-0
• UNSPSC Code: 12352202
• EC Number: 234-318-8
• NACRES: NA.75
• MDL number: MFCD00130536
• eCl@ss: 34058011

Propiedades

• Quality Segment: 200
• sterility: γ-irradiated
• product line: BioXtra
• assay: ≥98%
• form: powder
• mol wt: 76-81 kDa
• concentration: ~25 mM
• technique(s): cell culture | mammalian: suitable
• impurities: HIV and HBsAg, source material tested negative, iron, essentially free
• solubility: H₂O: 50 mg/mL
• UniProt accession no.: P02787
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TF(7018)

Descripción

General description

apo-Transferrin (aTF) human can be supplemented with iron or used to bind free iron present in media. It can be used to transport iron into mammalian cells.

Application

apo-Transferrin human has been used as a supplement:

• in Dulbecco′s modified Eagle medium/HamF12 (DMEM/F-12) to culture human adrenocortical carcinoma cells[1]
• in DMEM/F-12 to culture neural precursor cells from mice[2]
• in collecting medium NCSU23 to culture preantral follicles from porcine ovaries[3]

Biochem/physiol Actions

apo-Transferrin (aTF) is an endogenous iron chelator and shows protective effects in the host by scavenging free iron and preventing the formation of toxic reactive oxygen species (ROS). It shows a therapeutic effect against thalassemia.[4] After the release of iron, receptor-bound apo-transferrin reaches the cell surface instead of being transported to lysosomes for degradation. The cell surface has a neutral pH that promotes the release of apo-transferrin from its receptor, where it can again circulate and bind additional iron to undergo further rounds of iron delivery to cells.

Other Notes

Proteína transportadora de hierro no hemo.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3