Saltar al contenido
Merck
Todas las fotos(1)

Documentos

S8015

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

grado

for molecular biology

formulario

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

actividad extraña

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temp. de almacenamiento

room temp

Descripción general

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Aplicación

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Características y beneficios

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Componentes

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Producto relacionado

Referencia del producto
Descripción
Precios

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

D0428

1 kb DNA Ladder, for DNA electrophoresis

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

T9285

Tampón Tris-EDTA, 100X concentrate for Northern and Southern blotting

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

B6185

Bionic™ Buffer, 10X Concentrate, for Northern and Southern blotting, solution

GERPN3005

ECL™ Direct Nucleic Acid, Cytiva RPN3005, pack of 1 ea

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

A J Bogers et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2(3), 160-166 (1988-01-01)
To identify risk factors for hospital mortality after postinfarction left ventricular aneurysmectomy (PILVA), 83 consecutive cases of PILVA were studied in a retrospective analysis. Hospital mortality was 14.4%. Most of the factors correlated with hospital mortality were associated with poor
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico