P1903

Pyruvate Kinase from Bacillus stearothermophilus

Type VIII, lyophilized powder, 100-300 units/mg protein

• Sinónimos: ATP:pyruvate 2-O-phosphotransferase, PK
• Número CAS: 9001-59-6
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-616-2
• NACRES: NA.54
• MDL number: MFCD00081946
• Número CE: 2.7.1.40 (BRENDA, IUBMB)
• Specific activity: 100-300 units/mg protein
• Biological source: Bacillus sp. (B. stearothermophilus)

Propiedades

• biological source: Bacillus sp. (B. stearothermophilus)
• type: Type VIII
• form: lyophilized powder
• specific activity: 100-300 units/mg protein
• storage temp.: 2-8°C

Descripción

General description

Research Area: CELL SIGNALING
Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits organized in a dimer-of-dimers configuration. Four isoforms of pyruvate kinase exist in mammals, namely PKM1, PKM2, PKR, and PKL.[1] Pyruvate kinase can be found in both tetrameric and dimeric forms.

Application

Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.

Pyruvate Kinase from Bacillus stearothermophilus may be used in biofuel production.

Biochem/physiol Actions

Pyruvate Kinase from Bacillus stearothermophilus is activated by AMP and ribose 5-phosphate.

Pyruvate kinase is an enzyme that facilitates the conversion of phosphoenolpyruvate (PEP) and ADP to pyruvate and ATP in glycolysis and is involved in the regulation of cell metabolism.[1] It serves as a terminal enzyme in the glycolytic pathway. This reaction is favorable due to the high energy of hydrolysis of PEP.[1]
Pyruvate kinase is a key regulator controlling metabolic flux and ATP production in glycolysis and is considered a potential drug target. Additionally, pyruvate kinases are subject to regulation by heterotropic effectors, with fructose 1,6-bisphosphate (FBP) being the most widely known allosteric activator of bacterial, yeast, and mammalian enzymes. Furthermore, PKM2, one of the four isoforms of pyruvate kinase, is extensively expressed in various types of tumors and is associated with tumorigenesis.

Physical form

Lyophilized powder containing Tris buffer salts, pH 8.5

Analysis Note

Protein determined by biuret

Other Notes

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.2 at 30 °C.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)