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K0253

Keratin from human epidermis

aqueous solution

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Número CAS:
68238-35-7
UNSPSC Code:
12352202
NACRES:
NA.61
MDL number:
MFCD00131434
Form:
urea solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human epidermis
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biological source

human epidermis

Quality Level

200

assay

≥90% (SDS-PAGE)

form

urea solution

technique(s)

ELISA: suitable

UniProt accession no.

P04264

storage temp.

2-8°C

Gene Information

human ... KRT1(3848)

General description

Keratins are characteristic intermediate filament proteins of the epithelia. In human, keratin is classified into type I and type II. About 54 genes encodes for the keratin protein. The keratin 1 (KRT1) gene is mapped to human chromosome 12q13.13. It is the primary component of hair, skin and nails.

Application

Keratin from human epidermis has been used:
  • as an analyte and a cross-reactant in the antibody-immobilized chip for testing selectivity coefficients[1]
  • as a standard in extracellular matrix (ECM) protein in in vitro protein degradation assays.[2]
  • asa a standard in enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Keratins are important for the mechanical stability and integrity of epithelial cells and tissues. Some keratins also have regulatory functions and are involved in intracellular signaling pathways, e.g. protection from stress, wound healing, and apoptosis.[3] This diverse group of cytoskeletal scaffolding proteins form intermediate filament networks which provide structural support to keratinocytes that maintain the integrity of the skin. Expression of keratin genes is usually regulated by differentiation of the epidermal cells within the stratifying squamous epithelium.[4]

Physical form

Solution in 8 M urea, 50 mM Tris, 0.1 M β-mercaptoethanol and 0.1% sodium azide, pH 8.4

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Este artículo
WH0003848M1SAB4501653SAB5500125
Keratin from human epidermis aqueous solution

Sigma-Aldrich

K0253

Keratin from human epidermis

Monoclonal Anti-KRT1 antibody produced in mouse clone 2A7, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0003848M1

Monoclonal Anti-KRT1 antibody produced in mouse

Anti-Keratin 8 antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4501653

Anti-Keratin 8 antibody produced in rabbit

Anti-Keratin 17 antibody, Rabbit monoclonal clone SP95, recombinant, expressed in proprietary host, affinity isolated antibody

Sigma-Aldrich

SAB5500125

Anti-Keratin 17 antibody, Rabbit monoclonal

assay

≥90% (SDS-PAGE)

assay

-

assay

-

assay

-

biological source

human epidermis

biological source

mouse

biological source

rabbit

biological source

rabbit

technique(s)

ELISA: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

ELISA: 1:40000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

flow cytometry: 1:100, immunoblotting: 1:25, immunohistochemistry: 1:100

form

urea solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

UniProt accession no.

P04264

UniProt accession no.

P04264

UniProt accession no.

P05787

UniProt accession no.

Q04695

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000502380
0000502380
0000488475
0000494155
0000481482

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Número de artículo de comercio global

SKUGTIN
K0253-1MG04061826675557
K0253-5MG04061833262931
K0253-10MG04061833262924

Preguntas

  1. I need to ask about the supplied volume of this product. Also, can this product be used for fungal experimental studies as keratin is prepared in urea that might be toxic for cells?

    1 respuesta

    1. The volume of K0253 keratin derived from human epidermis is influenced by both the pack size and the lot-specific concentration. Here's a sample CofA for reference, which can be pulled from our website.
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/K0253-BULKSLCQ7891.pdf

      Also, keratin can be used for fungal experimental studies, but the presence of urea in its preparation may pose a concern depending on the concentration used. Urea can be cytotoxic at higher concentrations, potentially affecting fungal growth and experimental outcomes. To mitigate this, researchers often dialyze or extensively wash keratin preparations to remove residual urea. It is advisable to test the cytotoxicity of the prepared keratin on fungal cells before proceeding with experiments. Alternative keratin extraction methods that avoid urea may also be considered if cell viability is a major concern.
      K0253 is referenced in the study "Microbial Degradation of Native Keratin in Batch Fermentation".
      https://scialert.net/fulltext/fulltextpdf.php?pdf=ansinet/pjbs/1999/627-634.pdf

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