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I2911

IMDM

StableCell, with stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

Iscove’s Modified Dulbecco’s Medium, IMDM

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Tamaño de envaseSKUDisponibilidadPrecio
500 mL
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55,80 €

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UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:

55,80 €


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Nombre del producto

StableCell IMDM, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Este artículo
FG0465I3390I6529
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

components

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

components

stable glutamine: yes
NaHCO3: yes
glucose: yes
phenol red: yes

components

phenol red: yes
L-glutamine: no
glucose: yes

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient


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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Eleni Gelali et al.
Nature communications, 10(1), 1636-1636 (2019-04-11)
DNA fluorescence in situ hybridization (DNA FISH) is a powerful method to study chromosomal organization in single cells. At present, there is a lack of free resources of DNA FISH probes and probe design tools which can be readily applied.
Gustav Nilsonne et al.
Behavioral and brain functions : BBF, 7, 47-47 (2011-11-22)
Recent advances in cell biology and gene regulation suggest mechanisms whereby associative learning could be performed by single cells. Therefore, we explored a model of classical conditioning in human macrophages in vitro. In macrophage cultures, bacterial lipopolysaccharide (LPS; unconditioned stimulus)
Christine Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(25), 8691-8696 (2008-06-20)
A model of chemical thymectomy by inducible Rag ablation was used to study peripheral T cell homeostasis. Induction of Rag ablation was efficient and complete, leading to cessation of thymic T cell production within 3-4 weeks. The decay of peripheral



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SKUGTIN
I2911-500ML04061833848760

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