C9205

Anti-proteína gliofibrilar ácida (GFAP) monoclonal antibody produced in mouse

clone G-A-5, purified from hybridoma cell culture

• Sinónimos: Anti-GFAP
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: CY3 conjugate
• Clone: G-A-5, monoclonal
• Application: IF (d)
• Citations: 236

Propiedades

• biological source: mouse
• Quality Segment: 200
• conjugate: CY3 conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: G-A-5, monoclonal
• form: buffered aqueous solution
• species reactivity: human, pig, rat
• technique(s): direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections
• isotype: IgG1
• UniProt accession no.: P14136
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... GFAP(2670) ; rat ... Gfap(24387)

Descripción

General description

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Immunogen

GFAP de médula espinal de cerdo

GFAP from pig spinal cord

Application

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in

• immunohistochemistry[1]
• immunoblotting
• immunolabelling,
• immunocytochemistry
• immunofluorescence

Biochem/physiol Actions

El anticuerpo reacciona específicamente con la GFAP en análisis de inmunotransferencia y marca los astrocitos, las células de la glía de Bergmann y los condrocitos del cartílago elástico en la tinción inmunohistoquímica. El anticuerpo reacciona con el antígeno glial específico en cortes de tejido congelados o fijados en alcohol.

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.

The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)