Saltar al contenido
Merck
Todas las fotos(1)

Documentos

C5736

Sigma-Aldrich

Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse

clone 15C10, purified immunoglobulin, buffered aqueous glycerol solution

Sinónimos:

Anti-CANP, Anti-CANP1, Anti-CANPL1, Anti-SPG76, Anti-muCANP, Anti-muCL

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número MDL:
MFCD03455420
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

15C10, monoclonal

formulario

buffered aqueous glycerol solution

reactividad de especies

bovine, mouse, rat, human

técnicas

immunoprecipitation (IP): 2-5 μL (native and denaturing conditions)
indirect ELISA: suitable
western blot (chemiluminescent): 1:1,000

isotipo

IgG1

Nº de acceso UniProt

P07384

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CAPN1(823) , CAPNS1(826)
mouse ... Capn1(12333) , Capns1(12336)
rat ... Capn1(29153) , Capns1(29156)

Descripción general

Calpain-1 (CAPN1), an intracellular, calcium-dependent cysteine protease, has four domains, such as, the N-terminal anchor helix region, the CysPc protease domain, the C2 domain-like domain and the penta-EF-hand domain (PEF). CAPN1 codes for μ-calpain protein. CAPN1 gene is abundantly expressed in the central nervous system (CNS). This gene is located on human chromosome 11q13.1.

Especificidad

Specifically recognizes the 80 kDa subunit of μ-calpain as well as two smaller proteins that are presumed to be degradation products.

Inmunógeno

purified human μ-calpain from placenta (calpain-I, protein kinase-C activating factor, E.C. 3.4.22.17).

Aplicación

Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse has been used in:
  • western blotting
  • co-immunoprecipitation
  • immunofluorescence
  • immunofluorescence staining

Acciones bioquímicas o fisiológicas

Calpain-1 (CAPN1) plays a key role in microtubular regulation, cerebellar development, synaptic plasticity, synaptic restructuring, axon maturation and maintenance. CAPN1 activation is essential to provide a neuro-protective role in central nervous system (CNS). Mutations on CAPN1 gene results in spastic paraplegia 76 (SPG7), a complicated form of hereditary spastic paraplegia (HSP).

Forma física

Solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, and 3 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Fang Peng et al.
Orphanet journal of rare diseases, 14(1), 83-83 (2019-04-27)
Hereditary spastic paraplegias (HSP) are of great clinical and genetic heterogeneity. According to the clinical features, HSP can be divided into pure or complicated subtypes which combined with other neurological symptoms including cerebellar ataxia. Up to date, 78 loci or
Monica Averna et al.
Biochimica et biophysica acta, 1812(12), 1649-1657 (2011-10-11)
We are here reporting that in peripheral blood mononuclear cells (PBMC) of patients homozygous for F508del-CFTR the calpain-calpastatin system undergoes a profound alteration. In fact, calpain basal activity, almost undetectable in control PBMC, becomes measurable at a significant extent in
Christian Giordano et al.
PloS one, 10(6), e0131068-e0131068 (2015-06-25)
Short-term intermittent hypoxia (IH) is common in patients with acute respiratory disorders. Although prolonged exposure to hypoxia induces atrophy and increased fatigability of skeletal muscle, the response to short-term IH is less well known. We hypothesized that the diaphragm and
Wei Cui et al.
Cell & bioscience, 5, 36-36 (2015-07-07)
Under inflammatory conditions or during tumor progression macrophages acquire distinct phenotypes, with factors of the microenvironment such as hypoxia and transforming growth factor β (TGFβ) shaping their functional plasticity. TGFβ is among the factors causing alternative macrophage activation, which contributes
Aakash Shetty et al.
European journal of medical genetics, 62(12), 103605-103605 (2018-12-21)
To characterize the phenotype of CAPN1 (SPG76) mutations in patients diagnosed with hereditary spastic paraplegia (HSP). The CAPN1 gene, located on chromosome 11q13.1, is a protein-coding gene involved in neuronal plasticity, migration, microtubular regulation and cerebellar development. Several families with
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico