C2230

Coelenterazine, native

solid

• Sinónimos: 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one, CLZN
• Fórmula empírica (notación de Hill): C₂₆H₂₁N₃O₃
• Número de CAS: 55779-48-1
• Peso molecular: 423.46
• Número MDL: MFCD00467176
• Código UNSPSC: 12352200
• ID de la sustancia en PubChem: 24892477
• NACRES: NA.32

Propiedades

• Ensayo: ≥95% (TLC)
• Formulario: solid
• solubilidad: methanol and ethanol: soluble
• temp. de almacenamiento: −20°C
• cadena SMILES: Oc1ccc(CC2=NC3=C(Cc4ccccc4)NC(=CN3C2=O)c5ccc(O)cc5)cc1
• InChI: 1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
• Clave InChI: YHIPILPTUVMWQT-UHFFFAOYSA-N

Descripción

Descripción general

Coelenterazine is a luciferin molecule, which is utilized by several luciferases, such as Renilla, Oplophorus and Periphylla.[1]

Aplicación

Coelenterazine, native was used:-

• For Aequorin assays[2]
• For measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cells[3]
• In Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity[4]

Acciones bioquímicas o fisiológicas

Luminophore of the aequorin complex used to measure Ca²⁺ concentration.

Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca²⁺ binds to the complex; used to measure Ca²⁺ concentration in cells with high sensitivity and large dynamic range.

Precaución

Protect from exposure to light

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)