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B8291

BSK-H Medium, Complete

With 6% rabbit serum, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

Barbour-Stoenner-Kelly medium, Borrelia burgdorferi medium

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Tamaño de envaseSKUDisponibilidadPrecio
100 mL
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165,00 €
500 mL
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460,00 €
1 L
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462,00 €

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UNSPSC Code:
12352207
NACRES:
NA.75

165,00 €


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Nombre del producto

BSK-H Medium, Complete, sterile-filtered, with 6% rabbit serum, suitable for Borrelia burgdorferi

Quality Segment

sterility

sterile-filtered

form

liquid

suitability

suitable for Borrelia burgdorferi

application(s)

food and beverages

components

NaHCO3: 2.068 g/L
phenol red: 0.01997 g/L
glucose: 5.64 g/L (Dextro)
HEPES: 5.64 g/L

shipped in

dry ice

storage temp.

−20°C

Application

Complete BSK-H Medium was used to culture B. burgdorferi to study the pathology of lyme borreliosis.[1][2] It may be used in serum supplemented formulation for the growth and expansion of the Lyme desease spirochete, Borrelia burgdorferi.

Biochem/physiol Actions

BSK-H medium is a standardized complex medium designed to support the growth of the Lyme disease spirochete, Borrelia burgdorferi. To standardize the procedure for isolating and culturing Lyme disease spirochetes, Pollack, RJ et. al. modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow related spirochetes including Borrelia burgdorferi N40, Guilford, and JD-1 as well as strains of Borrelia hermsii (HS-1) and of Borrelia coriaceae (CO53). Standardized medium, supplemented with prescreened rabbit serum, facilitates comparison of research results between laboratories and may eventually permit definitive clinical diagnosis of Lyme disease based on demonstration of the pathogen. The standardized medium is designated BSK-H.
Barbour-Stonner-Kelly (BSK) medium is a modified medium for the culture of Borrelia strains that cause lyme borreliosis. BSK medium is a cheaper alternative for the selective culture of B. burgdorferi s.l. strains but not B. afzelii strain.[3]

Preparation Note

BSK-H Medium is supplied as a sterile-filtered liquid. After thawing, mix well by inverting the bottle prior to use. If the thawed medium will not be used within a few days it is recommended that the medium be refrozen in working aliquots to avoid repeated free-thaw cycles. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.

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1 of 1

Este artículo
B3528S014651417C
suitability

suitable for Borrelia burgdorferi

suitability

suitable for Borrelia burgdorferi (Qualified)

suitability

-

suitability

-

sterility

sterile-filtered

sterility

sterile; sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

application(s)

food and beverages

application(s)

food and beverages

application(s)

-

application(s)

-

components

NaHCO3: 2.068 g/L
phenol red: 0.01997 g/L
glucose: 5.64 g/L (Dextro)
HEPES: 5.64 g/L

components

NaHCO3: 2.2 g/L
glucose: 6.0 g/L (Dextro)
phenol red: 0.02124 g/L
HEPES: 6.0 g/L

components

NaHCO3: 0.4 g/L
glucose: 2 g/L (Dextro)
L-glutamine: 1.8 g/L

components

L-glutamine: 292 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes
NaHCO3: 840 mg/L
HEPES: 4766.4 mg/L

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C


Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

64-17-5

CAS No.


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Nataliia Rudenko et al.
Journal of clinical microbiology, 46(10), 3540-3543 (2008-07-25)
Molecular analysis of a clinical sample confirmed the presence of Borrelia bissettii DNA in cardiac valve tissue from a patient with endocarditis and aortic valve stenosis. This evidence strongly supports the involvement of B. bissettii in Lyme disease in Europe.
Jana Tuhácková et al.
Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia, 149(2), 297-302 (2006-04-08)
Verification of the efficacy of Biocan B inj. ad us. vet. (Bioveta, a.s.) was done by challenge testing. Ticks collected in the nature were used as natural vectors of the infection. Six beagles and two control ones were used in
Zachary A Schiller et al.
The Journal of clinical investigation, 131(11) (2021-04-30)
Disrupting transmission of Borrelia burgdorferi sensu lato complex (B. burgdorferi) from infected ticks to humans is one strategy to prevent the significant morbidity from Lyme disease. We have previously shown that an anti-OspA human mAb, 2217, prevents transmission of B.



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SKUGTIN
B8291-500ML04061833442685
B8291-100ML04061833442661
B8291-1L04061833442678

Preguntas

  1. Is rabbit serum inactivated by heat? Thanks

    1 respuesta
    1. No, this product has not been heat inactivated.

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