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A4914

IgG anti-conejo (molécula completa)-Peroxidasa antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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A ustedes/SKUDisponibilidadPrecio
1 mL
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176,00 €

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
129

176,00 €


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biological source

goat

Quality Segment

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit polyclonal IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary polyclonal anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the immunological analysis of target proteins. Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is specific for all rabbit IgGs and does not bind to human serum proteins in ELISA.

Immunogen

Purified rabbit IgG

Application

Se realizaron análisis de inmunoprecipitación y inmunoelectrotransferencia (técnica western) de los lisados celulares C33A utilizando IgG anti-conejo de cabra conjugada con HRP como el anticuerpo secundario.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used in sandwich ELISA (diluted 1:3000 in PBS-0.05% Tween 20) and immunoelectrophoresis. The product can also be used for western blotting applications (at 1:2000 dilutions in mouse liver homogenates , or in mouse embryonic fibroblasts ).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este artículo
A6154A8275A9169
conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3



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