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A0480

Sigma-Aldrich

Anti-Actin (plant) antibody, Mouse monoclonal

clone 10-B3 (MAbGPa), purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-Actin (plant) antibody produced in mouse

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About This Item

Número MDL:
MFCD00145889
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

10-B3 (MAbGPa), monoclonal

mol peso

antigen ~45 kDa

reactividad de especies

plant

envase

antibody small pack of 25 μL

técnicas

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using corn leaves extracts

isotipo

IgG2b

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Arabidopsis thaliana ... ACT8(841347)

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Descripción general

Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).
Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.

Inmunógeno

ACT8 protein.

Aplicación

Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:
  • two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
  • immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
  • immunoblotting

Acciones bioquímicas o fisiológicas

In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Hong-Xian Hsing et al.
Botanical studies, 57(1), 30-30 (2017-06-10)
Phalaenopsis orchid (Phal. orchid) is visually attractive and it is important economic floriculture species. Phal. orchids have many unique biological features. However, investigation of these features and validation on their biological functions are limited due to the lack of an
Jodi L Stewart et al.
PloS one, 6(5), e19894-e19894 (2011-06-01)
As photoautotrophs, plants can use both the form and amount of fixed carbon as a measure of the light environment. In this study, we used a variety of approaches to elucidate the role of exogenous sucrose in modifying seedling growth
Y Q An et al.
The Plant journal : for cell and molecular biology, 10(1), 107-121 (1996-07-01)
Arabidopsis has a complex and ancient actin gene family encoding six divergent subclasses of proteins. One subclass is represented by ACT2 and ACT8, which encode nearly identical proteins. These two genes differ significantly in flanking and intron sequences and in
An Endogenous Carbon-Sensing Pathway Triggers Increased Auxin Flux and Hypocotyl Elongation
Lilley JL, et al.
Plant Physiology, 160(4), 2261-2270 (2012)
Paolo Longoni et al.
Plant physiology, 169(4), 2874-2883 (2015-10-07)
Light-harvesting complex II (LHCII) is a crucial component of the photosynthetic machinery, with central roles in light capture and acclimation to changing light. The association of an LHCII trimer with PSI in the PSI-LHCII supercomplex is strictly dependent on LHCII
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