it is one drop of chromogen to 1ml of substrate buffer.
957D
DAB Substrate Kit
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NACRES:
NA.32
UNSPSC Code:
12161503
Technique(s):
immunohistochemistry: suitable
Storage temp.:
2-8°C
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Servicio técnico
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Permítanos ayudarledescription
For In Vitro Diagnostic Use in Select Regions
Quality Segment
form
aqueous solution
packaging
pkg of 1000 tests (957D-60), pkg of 150 tests (957D-50), pkg of 2000 tests (957D-30), pkg of 500 tests (957D-20), pkg of 5000 tests (957D-40)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry: suitable
storage temp.
2-8°C
General description
DAB Substrate Kit is intended for use in immunohistochemistry (IHC) staining protocols. This reagent is designed to detect target antigens in formalin-fixed, paraffin-embedded tissue sections when used in conjunction with primary antibodies and appropriate secondary antibody conjugates in the IHC staining process.When in the presence of HRP polymer, DAB produces a brown precipitate that can be readily visualized using light microscopy. Kits contain: DAB chromogen and DAB buffer substrate.
Physical form
Ready to use reagent
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Analysis Note
United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO
Canada - IVD
European Union - IVD
Japan - RUO
Other Notes
For Technical Service please contact: 800-665-7284 or email: [email protected]
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Muta. 2
Clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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how to prep DAB?
1 respuesta-
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What is the volume for "1 drop" of DAB chromogen?
1 respuesta-
The volume used to prepare 1ml of DAB would be 0.006mls, rounded to 0.01mls based on calculations for the number of tests in the kit 957D-20.
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Is the absence of color normal when 1 drop of chromagen and 1 ml of substrate are used, given the usual light purple color?
1 respuesta-
Ensure thorough mixing of the chromagen before combining. The color outcome is influenced by the chromogen's age. A lack of color typically suggests very recent or fresh DAB, which has not undergone oxidation.
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