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73353

Atto 647N iodoacetamide

BioReagent, suitable for fluorescence

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UNSPSC Code:
12352200


product line

BioReagent

form

solid

mol wt

Mw 969 g/mol

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 644 nm; λem 661 nm

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 647N is a superior label with high molecular absorption (150.000) and quantum yield (0.65) as well as sufficient stoke′s shift. Atto 647 N is characterized by a high thermal and photostability.
Absorption and fluorescence are independent of pH, at least in the most relevant range of pH 4 to 11. The iodoacetamide derivative reacts, like the maleimide, with a sulfhydryl group forming a thioether bond. It is predominantly used for tagging cystein residues of proteins.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.


Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Volker Westphal et al.
Science (New York, N.Y.), 320(5873), 246-249 (2008-02-23)
We present video-rate (28 frames per second) far-field optical imaging with a focal spot size of 62 nanometers in living cells. Fluorescently labeled synaptic vesicles inside the axons of cultured neurons were recorded with stimulated emission depletion (STED) microscopy in
S E D Webb et al.
Optics express, 16(25), 20258-20265 (2008-12-10)
We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B., et al.
Optical Nanoscopy, 1, 3-3 (2012)



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