47614

Fluorescamine

BioReagent, suitable for fluorescence, ≥99.0% (UV)

• Sinónimos: Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione
• Fórmula empírica (notación de Hill): C₁₇H₁₀O₄
• Número CAS: 38183-12-9
• Peso molecular: 278.26
• EC Number: 253-814-5
• UNSPSC Code: 12352200
• PubChem Substance ID: 329757329
• Beilstein/REAXYS Number: 921143
• MDL number: MFCD00005928

Propiedades

• product line: BioReagent
• assay: ≥99.0% (UV)
• mp: 153-157 °C
• solubility: acetonitrile: soluble, ethanol: soluble
• fluorescence: λ_ex 234 nm, λ_ex 390 nm; λ_em 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
• suitability: suitable for fluorescence
• SMILES string: O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
• InChI: 1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
• InChI key: ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Descripción

Application

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

Información de seguridad

• Clase de almacenamiento: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)