3023
AptaFluor® SAH Methyltransferase Assay
Sinónimos:
Methyltransferase Activity Assay
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
NACRES:
NA.84
UNSPSC Code:
41122100
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleshipped in
dry ice
storage temp.
−70°C
General description
The AptaFluor® SAH Methyltransferase TR-FRET Assay is a universal assay for enzymes that convert S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH), with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay can be used for all enzymes within the histone methyltransferase (HMT) and DNA methyltransferase (DNMT) families. The assay is designed specifically for high-throughput screening (HTS), with a simple mix-and-read format and 2 liquid addition steps. It is run in an endpoint mode: methyltransferase (MT) enzyme reactions are quenched with a universal stop reagent (provided) and then the detection reagents are added.
The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
View full Transcreener® product list
The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
- Simple, mix-and-read format ideal for HTS.
- Robust MT detection under initial velocity conditions (=20% conversion of SAM to SAH).
- Accommodates SAM concentrations as low as 100 nM and as high as 5 μM.
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering.
View full Transcreener® product list
Physical form
Kit with buffered aqueous solutions
Preparation Note
3023-B = 100 assay, 96-well
3023-1K = 1,000 assay, 384-well
3023-1K = 1,000 assay, 384-well
Legal Information
AptaFluor is a registered trademark of BellBrook Labs
Transcreener is a registered trademark of BellBrook Labs
1 of 1
Este artículo | |||
|---|---|---|---|
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
L Latini et al.
Cell death & disease, 5, e1404-e1404 (2014-09-05)
Spinal cord injury (SCI) is a devastating condition of CNS that often results in severe functional impairments for which there are no restorative therapies. As in other CNS injuries, in addition to the effects that are related to the primary
Kalyan C Kondapalli et al.
The Journal of biological chemistry, 287(43), 36239-36250 (2012-09-06)
Human NHA2, a newly discovered cation proton antiporter, is implicated in essential hypertension by gene linkage analysis. We show that NHA2 mediates phloretin-sensitive Na(+)-Li(+) counter-transport (SLC) activity, an established marker for hypertension. In contrast to bacteria and fungi where H(+)
Aileen K Sewell et al.
iScience, 25(5), 104186-104186 (2022-04-29)
The protein kinase complex target of rapamycin complex 1 (TORC1) is a critical mediator of nutrient sensing that has been widely studied in cultured cells and yeast, yet our understanding of the regulatory activities of TORC1 in the context of
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 229164-5G | 04061832339054 |
| 229164-25G | 04061832339047 |
| 3023-B | 04061835357444 |
| 3023-1K | 04061835357116 |