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E7889

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt solution

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Sinónimos:

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

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About This Item

Fórmula lineal:
[-CH2N(CH2CO2Na)CH2CO2H]2
Número de CAS:
139-33-3
Peso molecular:
336.21
Beilstein:
3822669
Número MDL:
MFCD00070672
Código UNSPSC:
12352107
ID de la sustancia en PubChem:
24894660
NACRES:
NA.31

grado

for molecular biology

Nivel de calidad

300

esterilidad

0.2 μm filtered

formulario

liquid

idoneidad de la reacción

reagent type: chelator

concentración

0.5 M in H2O

pH

7.5-8.5

idoneidad

suitable for molecular biology

aplicaciones

microbiology

actividad extraña

DNase, RNase, NICKase and protease, none detected

cadena SMILES

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

Clave InChI

ZGTMUACCHSMWAC-UHFFFAOYSA-L

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Descripción general

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Aplicación

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Características y beneficios

molecular biology grade

Nota de preparación

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Otras notas

For additional information on our range of Biochemicals, please complete this form.

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Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Warning

Frases de peligro

H373

Consejos de prudencia

P260 - P314 - P501

Clasificaciones de peligro

STOT RE 2 Inhalation

Órganos de actuación

Respiratory Tract

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000384159
0000387324
0000392905
0000390872
0000364461

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The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
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Foods (Basel, Switzerland), 10(1) (2021-01-21)
Isothiocyanates (ITCs) are important functional components of cruciferous vegetables. The principal isothiocyanate molecule in broccoli is sulforaphane (SFN), followed by erucin (ERN). They are sensitive to changes in temperature, especially high temperature environments where they are prone to degradation. The
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Science (New York, N.Y.), 371(6527) (2021-01-23)
The skin confers biophysical and immunological protection through a complex cellular network established early in embryonic development. We profiled the transcriptomes of more than 500,000 single cells from developing human fetal skin, healthy adult skin, and adult skin with atopic
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