MAB030
Anti-DNA Antibody, double stranded, clone BV16-13
culture supernatant, clone BV16-13, Chemicon®
Sinónimos:
Double-stranded DNA antibody
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
culture supernatant
tipo de anticuerpo
primary antibodies
clon
BV16-13, monoclonal
reactividad de especies (predicha por homología)
all
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable
isotipo
IgG2aκ
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Especificidad
Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.
BINDING ASSAY:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ANTIGEN BINDING
PROFILE: Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
BINDING ASSAY:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ANTIGEN BINDING
PROFILE: Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
Inmunógeno
Epitope: double stranded, 28% reactive with single stranded
Aplicación
Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
EIA/RIA
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Apoptosis & Cancer
Epigenetics & Nuclear Function
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Apoptosis - Additional
Cell Cycle, DNA Replication & Repair
Apoptosis - Additional
Forma física
Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Almacenamiento y estabilidad
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Herramienta de selección de productos.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Rajeeva Singh et al.
Molecular pharmaceutics, 17(1), 50-58 (2019-11-20)
DNA-targeting indolinobenzodiazepine dimer (IGN) payloads are used in several clinical-stage antibody-drug conjugates. IGN drugs alkylate DNA through the single imine moiety present in the dimer in contrast to the pyrrolobenzodiazepine dimer drugs, such as talirine and tesirine, which contain two
Sravani K Ramisetty et al.
International journal of biological macromolecules, 103, 845-853 (2017-05-26)
The macromolecules of the bacterial cell occupy 20-40% of the total cytosol volume, and crowded environments have long been known to compact and stabilize DNA. Nevertheless, investigations on DNA-protein binding are generally performed in the absence of crowding, which may
Petter Langlete et al.
Frontiers in microbiology, 10, 2708-2708 (2019-12-12)
Extracellular vesicles secreted by Gram-negative bacteria have proven to be important in bacterial defense, communication and host-pathogen relationships. They resemble smaller versions of the bacterial mother cell, with similar contents of proteins, LPS, DNA, and RNA. Vesicles can elicit a
Feng Huang et al.
Molecular medicine reports, 16(4), 4817-4822 (2017-08-10)
Lupus nephritis (LN) is a common complication of systemic lupus erythematosus. The present study aimed to elucidate the protective effect of magnolol (MG) on the progression of LN, via inhibition of key signaling pathways. The results of the present study
C A Cooke et al.
The Journal of cell biology, 120(5), 1083-1091 (1993-03-01)
The location of the cis-acting DNA sequences that direct the assembly of the mammalian kinetochore is not known. A variety of circumstantial evidence, however, has led to the widespread belief that they are present throughout the kinetochore including the kinetochore
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico