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AG310

Sigma-Aldrich

Fluoro-Jade® B

Sinónimos:

FJB

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702

Formulario

powder

mol peso

Mw 681 Da

fabricante / nombre comercial

Fluoro-Jade®

técnicas

immunofluorescence: suitable

fluorescencia

λex 480 nm; λem 525 nm

Descripción general

Fluoro-JadeⓇ B is a polyanionic fluorescein derivative which sensitively and specifically binds to degenerating neurons. It is a dark red power that has a green iridescence with excitation peak at 480 nm and emission peak at 525 nm. The filter used for visualizing Fluoro-JadeⓇ B is a fluorescein/FITC filter. Fluoro-JadeⓇ B can be used on most tissue section types and thicknesses including frozen, vibratomed cryostat or paraffin-embedded sections from 3-50 µm. Fluoro-JadeⓇ B is faster and more reliable than older methods (e.g. suppressed silver) for the unequivocal qualitative detection and quantitative measurement of both and fine scale neuronal degeneration.
N-terminal GST-tagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells

Aplicación

Appearance: Dark red powder with green iridescence.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Advertencia

Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.

Forma física

Filter System : Fluorescein / FITC
Solubility: Very soluble in water and bases, moderately soluble in alcohol and weak acids.

Almacenamiento y estabilidad

The powder should be stored well sealed at room temperature, preferable in a dessicator, due to its hygroscopic nature. The liquid stock solution (0.01%) in distilled water can be stored at 2-8°C for up to 3 months. The working solution (0.0004%) in 0.1% acetic acid should be prepared fresh and not be stored or reused.

Nota de análisis

This layer chromatography using cellulose plates and a solvent system of n-propinol, water, and ammonium hydroxide (6:5:2) revealed the presence of two fluorescent isomers and two trace non-fluorescent bands. No amount of fluorescein or Fluoro-Jade was present

Información legal

FLUORO-JADE is a registered trademark of Histo-Chem, Inc.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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