71259
Insect GeneJuice® Transfection Reagent
Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.
Sinónimos:
GeneJuice Transfection
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 0.3 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 195,00 € | |
| 1 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 248,00 € | |
| 10 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 1870,00 € |
Acerca de este artículo
NACRES:
NA.54
UNSPSC Code:
41106502
Form:
suspension
Technique(s):
transfection: suitable
195,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleQuality Level
form
suspension
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
transfection: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Insect GeneJuice<TMSYMBOL></TMSYMBOL> Transfection Reagent is a proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for cotransfection of transfer plasmids with linearized virus DNA for the production of recombinant baculoviruses. Insect GeneJuice is ideal for large-scale protein expression in suspension-culture transfections of Sf9 insect cells when using pIEx<TMSYMBOL></TMSYMBOL> and pBiEx<TMSYMBOL></TMSYMBOL> vectors, which contain the immediate early baculovirus promoter, IE1.
Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.
- When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 75,726 when other reagent used with Sf9, thus 1.7 fold difference.
- When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 11,952 when other reagent used with High Five, thus 11 fold difference.
- When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 31,897 when Insect Genejuice used with High Five, thus 4.1 fold difference.
Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.
Legal Information
GeneJuice is a registered trademark of EMD Biosciences, Inc., Madison, WI USA
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Este artículo | |||
|---|---|---|---|
| form suspension | form liquid | form - | form liquid (aqueous solution) |
| technique(s) transfection: suitable | technique(s) transfection: suitable | technique(s) transfection: suitable | technique(s) transfection: suitable |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| storage condition OK to freeze | storage condition OK to freeze | storage condition - | storage condition - |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
Clase de almacenamiento
12 - Non Combustible Liquids
Certificados de análisis (COA)
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 71259-3 | 07790788053123 |
| 71259-4 | 04055977270860 |
| 71259-5 | 04055977270877 |