491002
SAHM1
≥93% (HPLC), solid, Notch transcription factor inhibitor, Calbiochem®
Sinónimos:
Notch Transcription Factor Inhibitor, SAHM1, AcβA1a-ERLRRRI*LCR*HHST (*=S5)
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 1 mg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 389,00 € |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C94H162N36O23S · 7C2HF3O2 · 13H2O
Peso molecular:
3228.95
UNSPSC Code:
12352200
NACRES:
NA.77
389,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleNombre del producto
Notch Transcription Factor Inhibitor, SAHM1, Notch Transcription Factor Inhibitor, SAHM1, is a cell-permeable inhibitor that prevents assembly in the NOTCH1 trans-activation complex.
Quality Level
assay
≥93% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
off-white
solubility
DMSO: 50 mg/mL
shipped in
wet ice
storage temp.
−20°C
General description
A cell-permeable, stabilized hydrocarbon-stapled α-helical peptide that targets a critical protein-protein interface and prevents assembly in the NOTCH1 trans-activation complex. Soluble FITC-SAHM1 was found to bind the pre-formed RAMANK-CSL complex (Kd = 0.12 µM) competitively with MAML1, whereas the unmodified FITC-MAML1 (21-36) peptide bound the complex with markedly diminished affinity in an in vitro pull down assay. Treatment of KOPT-K1 cell lines with 20 µM of compound results in the suppression of NOTCH1 target genes, HES4, DTX1, and HES1. A consistent repressive effect on NOTCH1-activated gene expression by this compound was observed across a panel of human T-ALL cell lines, containing diverse mutant NOTCH1 alleles. In HeLa cells, this peptide also inhibited the expression of ICN1 (IC50 = 6.56 µM) dose-dependently in a luciferase reporter-gene assay, as well as that of β-lactamase (IC50<2.5 µM) in a second reporter-gene assay. Furthermore, treatment with this peptide inhibits NOTCH-mediated proliferation in cultured cells at 15 µM, and in a mouse model of T-ALL cells at 30 mg/kg twice daily.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Acetyl-βAla-ERLRRRI*LCR*HHST
* = Two S5 molecules (a non-natural alkenyl amino acid) stapled together with a double bond
* = Two S5 molecules (a non-natural alkenyl amino acid) stapled together with a double bond
Moellering, R.E., et al. 2009. Nature462, 182.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Este artículo | |||
|---|---|---|---|
| form solid | form solid | form solid | form solid |
| assay ≥93% (HPLC) | assay ≥98% (HPLC) | assay ≥95% (HPLC) | assay ≥95% (HPLC) |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level 100 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| solubility DMSO: 50 mg/mL | solubility DMSO: 50 mg/mL, brown | solubility DMSO: 10 mg/mL | solubility DMSO: 10 mg/mL |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Prashan De Zoysa et al.
Development (Cambridge, England), 147(17) (2020-08-12)
The role played by the Notch pathway in cardiac progenitor cell biology remains to be elucidated. Delta-like ligand 4 (Dll4), the arterial-specific Notch ligand, is expressed by second heart field (SHF) progenitors at time-points that are crucial in SHF biology.
Cooperative NF-I?B and Notch1 signaling promotes macrophage-mediated MenaINV expression in breast cancer.
Duran, et al.
Breast Cancer Research, 25, 37-37 (2023)
Ved P Sharma et al.
Nature communications, 12(1), 7300-7300 (2021-12-17)
Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe CSCs in live mice
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 491002-1MG | 04055977273427 |