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217570

Sigma-Aldrich

Anti-CD3 Mouse mAb (UCHT1)

liquid, >95% (immunoglobulin, SDS-PAGE), clone UCHT1

Sinónimos:

Anti-CD3 Antibody, CD3 Detection Antibody, Mouse Anti-CD3

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

UCHT1, monoclonal

Análisis

>95% (immunoglobulin, SDS-PAGE)

formulario

liquid

contiene

0.5 mg/mL gentamycin sulfate as preservative

reactividad de especies

human

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

isotipo

IgG1

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.
Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.

Inmunógeno

human thymocytes/Sezary T cells

Aplicación

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see application references)

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Otras notas

Thibault, G. and Bardos, P. 1995. J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol.11, 329.
This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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