16-282
Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate
clone 4G10®, from mouse
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 200 μL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 569,00 € |
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
4G10®, monoclonal
Technique(s):
immunoprecipitation (IP): suitable
Application:
IP
Citations:
2
569,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4G10®, monoclonal
species reactivity (predicted by homology)
all
technique(s)
immunoprecipitation (IP): suitable
isotype
IgG2bκ
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Immunogen
Phosphotyramine-KLH
Application
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Biochem/physiol Actions
This antibody recognizes tyrosine-phosphorylated proteins from all species
Physical form
20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
Format: Purified
Protein G-Sepharose Chromatography
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE
Note: DO NOT FREEZE
Analysis Note
100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).
(Lane 2).
Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
Este artículo | |||
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone 4G10®, monoclonal | clone 4G10®, monoclonal | clone 4G10®, monoclonal | clone 4G10®, monoclonal |
| antibody form purified antibody | antibody form purified antibody | antibody form purified antibody | antibody form purified immunoglobulin |
| Gene Information human ... PID1(55022) | Gene Information human ... PID1(55022) | Gene Information - | Gene Information - |
| technique(s) immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
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M Whitfield et al.
Reproduction (Cambridge, England), 154(6), 827-842 (2017-10-04)
Lipid metabolism disorders (dyslipidemia) are causes of male infertility, but little is known about their impact on male gametes when considering post-testicular maturation events, given that studies concentrate most often on endocrine dysfunctions and testicular consequences. In this study, three-month-old
F Saez et al.
Andrology, 7(5), 654-661 (2019-06-05)
Lipid metabolic disorders (dyslipidemia) are constantly increasing in occidental societies and lead to the development of pathologies such as obesity, diabetes, and metabolic syndrome. It has been demonstrated that dyslipidemia can alter the reproductive function. Animal models have recently been
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 16-282 | 04053252641596 |